| MT

Melatonin, an indoleamine, is a natural compound produced by various organisms, including bacteria and eukaryotes.This compound was later identified as a hormone secreted in the brain during the night, playing a crucial role in regulating the sleep-wake cycle, also known as the circadian rhythm, in vertebrates.In vertebrates, melatonin's functions extend to synchronizing sleep-wake cycles, encompassing sleep-wake timing and blood pressure regulation, as well as controlling seasonal rhythmicity (circannual cycle), which includes reproduction, fattening, molting, and hibernation.Its effects are mediated through the activation of melatonin receptors and its role as an antioxidant.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	0.19	0.24	0.31	0.55	0.97	1.38	1.96	2.52

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Bovine MT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-102	97
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Bovine MT and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-97	90-110
	Average (%)	91	94	96
1 : 4	Range (%)	89-104	87-108	105-115
	Average (%)	93	98	110